The proteins encoded by viruses can affect the cell in more ways than one. Thus, it would be interesting to study the long term effects of trans-acting factors encoded by the viruses on cells. One approach may be to integrate the virus's genome into that of the cell's like how a normal virus would infect a cell. The problem is that the virus may integrate in a location within genome whereby the integrated viral DNA element may affect the function and expression of neighbouring genes. In the end, our observations may be affected. I was wondering if it is possible to introduce an artifical chromosome with an integrated viral element instead. It would allow us to study the effects of trans-acting elements encoded by the virus on cells' functions and the long term effect on the cell.
Subscribe to:
Post Comments (Atom)
3 comments:
Hey Dr. Dee,
Do you know what are the limits of pH and ionic concentration(conductivity) for both Human Serum Albumin and Human Immunoglobulin G before they are denatured?
Need it for something.
Cheers,
Ray
Dear Ray:
You can check this link for human serum albumin http://www.abcam.com/index.html?datasheet=8033. However, they are optimum conditions for storage.
Yours sincerely,
Dr Dee
For the antibodies, I would advise you to check out the company producing it. I would show you one example. You can check this link http://www.researchd.com/miscabs/trk4t24.htm
Yours sincerely,
Dr Dee
Post a Comment